UNIT 22.2 Preparation of Cytogenetic Specimens from Tissue Samples
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Bayani, J. and Squire, J. A. 2004. Preparation of Cytogenetic Specimens from Tissue Samples. Current Protocols in Cell Biology. 23:22.2:22.2.1–22.2.15.
- Published Online: 1 SEP 2004
- Published Print: JUN 2004
Cytogenetic specimens are prepared from short-term primary cultures established from tissue samples or from established cell lines. Cultures that are (80% confluent are treated with Colcemid to enrich for cells in mitosis. Cells are then swollen hypotonically and fixed before slides are prepared. Preparing good metaphase spreads is technically challenging, This unit provides a good discussion of the factors that go into making a good preparation.
- cell culture;
- cytogenetic preparations;
- metaphase spreads;
- tissue samples