Unit

UNIT 22.2 Preparation of Cytogenetic Specimens from Tissue Samples

  1. Jane Bayani,
  2. Jeremy A. Squire

Published Online: 1 SEP 2004

DOI: 10.1002/0471143030.cb2202s23

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Bayani, J. and Squire, J. A. 2004. Preparation of Cytogenetic Specimens from Tissue Samples. Current Protocols in Cell Biology. 23:22.2:22.2.1–22.2.15.

Author Information

  1. University of Toronto, Ontario, Canada

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: JUN 2004

Abstract

Cytogenetic specimens are prepared from short-term primary cultures established from tissue samples or from established cell lines. Cultures that are (80% confluent are treated with Colcemid to enrich for cells in mitosis. Cells are then swollen hypotonically and fixed before slides are prepared. Preparing good metaphase spreads is technically challenging, This unit provides a good discussion of the factors that go into making a good preparation.

Keywords:

  • cell culture;
  • cytogenetic preparations;
  • metaphase spreads;
  • tissue samples