Unit

UNIT 22.8 Detection of Mitotic Figures and Components of the Mitotic Machinery

  1. Jane Bayani,
  2. Jeremy A. Squire

Published Online: 1 JAN 2005

DOI: 10.1002/0471143030.cb2208s25

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Bayani, J. and Squire, J. A. 2005. Detection of Mitotic Figures and Components of the Mitotic Machinery. Current Protocols in Cell Biology. 25:22.8:22.8.1–22.8.6.

Author Information

  1. Princess Margaret Hospital and The Ontario Cancer Institute, Toronto, Ontario, Canada

Publication History

  1. Published Online: 1 JAN 2005
  2. Published Print: DEC 2004

Abstract

Mitotic figures have provided important information on the mechanisms behind observed chromosomal aberrations. Variations in cell culturing and fixation methods enhance the visualization of mitotic figures. Furthermore, immunohistochemistry using a host of antibodies against protein structures involved in the cell cycle, as well as against those components involved in anchoring chromosomal structures, can reveal changes in chromosomal segregation.

Keywords:

  • mitotic figures;
  • mitotic machinery;
  • immunohistochemistry;
  • chromosomal segregation