Unit

UNIT 22.13 Monitoring mRNA Export

  1. Kazuaki Tokunaga,
  2. Tokio Tani

Published Online: 1 DEC 2008

DOI: 10.1002/0471143030.cb2213s41

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Tokunaga, K. and Tani, T. 2008. Monitoring mRNA Export. Current Protocols in Cell Biology. 41:22.13:22.13.1–22.13.20.

Author Information

  1. Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto, Japan

Publication History

  1. Published Online: 1 DEC 2008
  2. Published Print: DEC 2008

Abstract

Transport of mRNA from the nucleus to the cytoplasm is an essential process for gene expression in eukaryotic cells. In this unit, methods for monitoring nuclear mRNA export are described. Visualization of cellular mRNAs by fluorescence in situ hybridization with oligo(dT) probes is effectively applied to monitoring mRNA export from the nucleus in yeast and mammalian cells. In addition to the protocols for fluorescence in situ hybridization, this unit includes an alternate method that the authors have been developing for visual analysis of nuclear mRNA export in living mammalian cells by microinjection of fluorescently labeled pre-mRNA into the nuclei. Curr. Protoc. Cell Biol. 41:22.13.1-22.13.20. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • mRNA;
  • transport;
  • in situ hybridization;
  • microinjection;
  • yeast;
  • mammalian cells