UNIT 22.14 Analysis of DNA Replication in Saccharomyces cerevisiae by Two-Dimensional and Pulsed-Field Gel Electrophoresis
Published Online: 1 DEC 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Ide, S. and Kobayashi, T. 2010. Analysis of DNA Replication in Saccharomyces cerevisiae by Two-Dimensional and Pulsed-Field Gel Electrophoresis. Current Protocols in Cell Biology. 49:22.14:22.14.1–22.14.12.
- Published Online: 1 DEC 2010
- Published Print: DEC 2010
Two methods to analyze DNA replication in budding yeast are described here. One is two-dimensional (2D) gel electrophoresis, which has been used to analyze replication intermediates in yeast. The other method is pulsed-field gel electrophoresis (PFGE) that monitors the replication status of a chromosome. These are convenient and easy, and they do not require specialized equipment. Curr. Protoc. Cell Biol. 49:22.14.1-22.14.12. © 2010 by John Wiley & Sons, Inc.
- two-dimensional gel electrophoresis;
- pulsed-field gel electrophoresis;
- DNA replication;
- agarose plug;
- replicative intermediate