Unit

UNIT 22.14 Analysis of DNA Replication in Saccharomyces cerevisiae by Two-Dimensional and Pulsed-Field Gel Electrophoresis

  1. Satoru Ide1,
  2. Takehiko Kobayashi1,2

Published Online: 1 DEC 2010

DOI: 10.1002/0471143030.cb2214s49

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Ide, S. and Kobayashi, T. 2010. Analysis of DNA Replication in Saccharomyces cerevisiae by Two-Dimensional and Pulsed-Field Gel Electrophoresis. Current Protocols in Cell Biology. 49:22.14:22.14.1–22.14.12.

Author Information

  1. 1

    National Institute of Genetics, Shizuoka, Japan

  2. 2

    The Graduate University for Advanced Studies, Sokendai, Mishima, Japan

Publication History

  1. Published Online: 1 DEC 2010
  2. Published Print: DEC 2010

Abstract

Two methods to analyze DNA replication in budding yeast are described here. One is two-dimensional (2D) gel electrophoresis, which has been used to analyze replication intermediates in yeast. The other method is pulsed-field gel electrophoresis (PFGE) that monitors the replication status of a chromosome. These are convenient and easy, and they do not require specialized equipment. Curr. Protoc. Cell Biol. 49:22.14.1-22.14.12. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • two-dimensional gel electrophoresis;
  • pulsed-field gel electrophoresis;
  • DNA replication;
  • agarose plug;
  • replicative intermediate