UNIT 23.9 Expanding Mouse Ventricular Cardiomyocytes Through GSK-3 Inhibition
Published Online: 2 DEC 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Buikema, J. W., Zwetsloot, P.-P. M., Doevendans, P. A., Sluijter, J. P. and Domian, I. J. 2013. Expanding Mouse Ventricular Cardiomyocytes Through GSK-3 Inhibition. Current Protocols in Cell Biology. 61:23.9:23.9.1–23.9.10.
- Published Online: 2 DEC 2013
Controlled proliferation of cardiomyocytes remains a major limitation in cell biology and one of the main underlying hurdles for true modern regenerative medicine. Here, a technique is described for robust expansion of early fetal-derived mouse ventricular cardiomyocytes on a platform usable for high-throughput molecular screening, tissue engineering and, potentially, in vivo translational experiments. This method provides a small-molecule approach to control proliferation or differentiation of early beating cardiomyocytes through modulation of the Wnt/β-catenin signaling pathway. Moreover, isolation and expansion of fetal cardiomyocytes takes less than 3 weeks, yields a relatively pure (∼70%) functional myogenic population, and is highly reproducible. Curr. Protoc. Cell Biol. 61:23.9.1-23.9.10. © 2013 by John Wiley & Sons, Inc.
- cardiomyocyte proliferation;
- GSK-3 inhibitor;
- Wnt/β-catenin signaling