UNIT 24.1 Using Fluorescent Sphingolipid Analogs to Study Intracellular Lipid Trafficking
Published Online: 1 JUN 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Singh, R. D., Marks, D. L. and Pagano, R. E. 2007. Using Fluorescent Sphingolipid Analogs to Study Intracellular Lipid Trafficking. Current Protocols in Cell Biology. 35:24.1:24.1.1–24.1.19.
- Published Online: 1 JUN 2007
- Published Print: JUN 2007
Sphingolipids (SLs), including glycosphingolipids, are found on the plasma membrane where they play important roles in a wide variety of cell functions, including cell-cell communication, cell growth and differentiation, host-pathogen interactions, and cell-signaling events. This unit illustrates the use of fluorescent SL analogs to identify the mechanisms underlying SL endocytosis and subsequent intracellular trafficking. Techniques used to study SL domain formation at the plasma membrane, endocytic mechanisms and intracellular transport steps are highlighted. The use of biochemical treatments and dominant-negative protein expression to block specific steps in lipid trafficking are also discussed.
- lipid trafficking