UNIT 25.2 Fabrication and Application of Nanofibrous Scaffolds in Tissue Engineering

  1. Wan-Ju Li1,2,
  2. Rocky S. Tuan1

Published Online: 1 MAR 2009

DOI: 10.1002/0471143030.cb2502s42

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Li, W.-J. and Tuan, R. S. 2009. Fabrication and Application of Nanofibrous Scaffolds in Tissue Engineering. Current Protocols in Cell Biology. 42:25.2:25.2.1–25.2.12.

Author Information

  1. 1

    National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland

  2. 2

    University of Wisconsin-Madison, Madison, Wisconsin

Publication History

  1. Published Online: 1 MAR 2009
  2. Published Print: MAR 2009


Nanofibers fabricated by electrospinning are morphological mimics of fibrous components of the native extracellular matrix, making nanofibrous scaffolds ideal for three-dimensional cell culture and tissue engineering applications. Although electrospinning is not a conventional technique in cell biology, the experimental setup may be constructed in a relatively straightforward manner, and the procedure can be carried out by individuals with limited engineering experience. Here, we detail a protocol for electrospinning of nanofibers and provide relevant specific details concerning the optimization of fiber formation (Basic Protocol 1). The protocol also includes conditions required for preparing biodegradable polymer solutions for the fabrication of nonwoven and aligned nanofibrous scaffolds suitable for various cell/tissue applications. In addition, information on effective cell loading into nanofibrous scaffolds and cellular constructs grown in a bioreactor is provided (Basic Protocol 2). Instructions for building the electrospinning apparatus are also included (see the Support Protocol). Curr. Protoc. Cell Biol. 42:25.2.1-25.2.12. © 2009 by John Wiley & Sons, Inc.


  • nanofiber;
  • electrospinning;
  • scaffold;
  • tissue engineering;
  • three-dimensional culture