Unit

UNIT 26.5 HIV-1 Interactions with Cells: From Viral Binding to Cell-Cell Transmission

  1. Alicia M. Janas,
  2. Li Wu

Published Online: 1 JUN 2009

DOI: 10.1002/0471143030.cb2605s43

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Janas, A. M. and Wu, L. 2009. HIV-1 Interactions with Cells: From Viral Binding to Cell-Cell Transmission. Current Protocols in Cell Biology. 43:26.5:26.5.1–26.5.20.

Author Information

  1. Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin

Publication History

  1. Published Online: 1 JUN 2009
  2. Published Print: JUN 2009

Abstract

Characterization of HIV-1 interactions with host cells is critical for cell biology studies of HIV-1. This unit describes a set of methods and protocols to perform quantitative assays of HIV-1 binding, internalization, infection, and cell-cell transmission. The protocols include: (1) generating infectious single-cycle or replication-competent HIV-1 stocks, (2) an HIV-1 binding and internalization assay, (3) HIV-1 infection of target cells and quantification of viral infection, and (4) HIV-1 cell-cell transmission assays. These functional assays provide useful tools to quantitatively study HIV-1 infection and viral transmission. Curr. Protoc. Cell Biol. 43:26.5.1-26.5.20. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • human immunodeficiency virus type 1 (HIV-1);
  • binding;
  • internalization;
  • infection;
  • viral transmission;
  • interactions