Unit

UNIT 26.8 Production of Lentiviral Vectors in Protein-free Media

  1. Hitoshi Kuroda1,
  2. Michael P. Marino2,
  3. Robert H. Kutner1,
  4. Jakob Reiser2

Published Online: 1 MAR 2011

DOI: 10.1002/0471143030.cb2608s50

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Kuroda, H., Marino, M. P., Kutner, R. H. and Reiser, J. 2011. Production of Lentiviral Vectors in Protein-free Media. Current Protocols in Cell Biology. 50:26.8:26.8.1–26.8.13.

Author Information

  1. 1

    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, Louisiana

  2. 2

    Division of Cellular and Gene Therapies, FDA/CBER, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAR 2011
  2. Published Print: MAR 2011

Abstract

The use of lentiviral vectors for transgene delivery in vitro and in vivo for applications in neuroscience, hematology, developmental biology, stem cell biology, and transgenesis has become commonplace. Lentiviral vectors provide an attractive toolfor transgene delivery in part because of their ability to incorporate(integrate)into the genomic DNA of target cells with high efficiency, especially in cells thatare not actively dividing. In addition, lentiviral vector-mediated transgeneexpression can be maintained for long periods of time. In this unit, we describe protocols for lentiviral vector production in protein-free media using polyethylenimine (PEI)-mediated transfection, resulting in consistent lentiviral vector stocks. Such stocks are then concentrated by ultracentrifugation. We also provide reliable QPCR protocols to titrate lentiviral vectors based on vector DNA copies present in genomic DNA extracted from transduced cells. The vector production and titration protocol described here can be completed within 8 days. Curr. Protoc. Cell Biol. 50:26.8.1-26.8.13. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • lentiviral vector production;
  • protein-free media;
  • PEI transfection