UNIT 26.10 Evaluation of Antiseptic Antiviral Activity of Chemical Agents

  1. Chloé Geller,
  2. Chantal Finance,
  3. Raphaël Emmanuel Duval

Published Online: 1 JUN 2011

DOI: 10.1002/0471143030.cb2610s51

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Geller, C., Finance, C. and Duval, R. E. 2011. Evaluation of Antiseptic Antiviral Activity of Chemical Agents. Current Protocols in Cell Biology. 51:26.10:26.10.1–26.10.22.

Author Information

  1. Nancy-University, CNRS, Nancy Cedex, France

Publication History

  1. Published Online: 1 JUN 2011
  2. Published Print: JUN 2011


Antiviral antisepsis and disinfection are crucial for preventing the environmental spread of viral infections. Emerging viruses and associated diseases, as well as nosocomial viral infections, have become a real issue in medical fields, and there are very few efficient and specific treatments available to fight most of these infections. Another issue is the potential environmental resistance and spread of viral particles. Therefore, it is essential to properly evaluate the efficacy of antiseptics-disinfectants (ATS-D) on viruses. ATS-D antiviral activity is evaluated by (1) combining viruses and test product for an appropriately defined and precise contact time, (2) neutralizing product activity, and (3) estimating the loss of viral infectivity. A germicide can be considered to have an efficient ATS-D antiviral activity if it induces a >3 or >4 log10 reduction (American and European regulatory agency requirements, respectively) in viral titers in a defined contact time. This unit describes a global methodology for evaluating chemical ATS-D antiviral activity. Curr. Protoc. Cell Biol. 51:26.10.1-26.10.22. © 2011 by John Wiley & Sons, Inc.


  • antiseptic;
  • antiviral;
  • chemical agents;
  • neutralization;
  • gel-filtration