Unit

UNIT 26.11 Rapid Titration of Viruses by Flow Cytometry

  1. Nir Drayman,
  2. Ariella Oppenheim

Published Online: 1 JUN 2011

DOI: 10.1002/0471143030.cb2611s51

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Drayman, N. and Oppenheim, A. 2011. Rapid Titration of Viruses by Flow Cytometry. Current Protocols in Cell Biology. 51:26.11:26.11.1–26.11.7.

Author Information

  1. Department of Hematology, Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel

Publication History

  1. Published Online: 1 JUN 2011
  2. Published Print: JUN 2011

Abstract

Traditionally, the most common methods used to titrate virus stocks are the plaque assay and the hemagglutination assay. The protocol presented here is based on the detection of viral-expressed proteins in infected cells by flow cytometry. It is simpler and more rapid than the traditional plaque-forming assay and it enables high-throughput analyses. Curr. Protoc. Cell Biol. 51:26.11.1-26.11.7. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • virus titration;
  • FACS;
  • SV40