Unit

UNIT 26.13 A Dual-Chamber Model of the Female Genital Tract to Evaluate Epithelial Toxicity of Candidate Anti-HIV Microbicides

  1. Kevin K. Ariën,
  2. Guido Vanham,
  3. Youssef Gali

Published Online: 1 SEP 2011

DOI: 10.1002/0471143030.cb2613s52

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Ariën, K. K., Vanham, G. and Gali, Y. 2011. A Dual-Chamber Model of the Female Genital Tract to Evaluate Epithelial Toxicity of Candidate Anti-HIV Microbicides. Current Protocols in Cell Biology. 52:26.13:26.13.1–26.13.17.

Author Information

  1. Virology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium

Publication History

  1. Published Online: 1 SEP 2011
  2. Published Print: SEP 2011

Abstract

Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides. Curr. Protoc. Cell Biol. 52:26.13.1-26.13.17. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • HIV;
  • sexual transmission;
  • microbicides;
  • toxicity;
  • female genital tract