Unit

UNIT 26.14 Methods for Growing and Titrating African Swine Fever Virus: Field and Laboratory Samples

  1. Angel L. Carrascosa,
  2. M. Jose Bustos,
  3. Patricia de Leon

Published Online: 1 DEC 2011

DOI: 10.1002/0471143030.cb2614s53

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Carrascosa, A. L., Bustos, M. J. and de Leon, P. 2011. Methods for Growing and Titrating African Swine Fever Virus: Field and Laboratory Samples. Current Protocols in Cell Biology. 53:26.14:26.14.1–26.14.25.

Author Information

  1. Centro de Biologia Molecular “Severo Ochoa” (CSIC-UAM), Universidad Autonoma de Madrid, Madrid, Spain

Publication History

  1. Published Online: 1 DEC 2011
  2. Published Print: DEC 2011

Abstract

Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and protocols to produce and titrate any ASFV strain in cell cultures. The procedures include (1) basic techniques to prepare virus-sensitive target cells; (2) strategies for growth, concentration, and purification of virus stocks; and (3) the semi-quantitative (end dilution) and quantitative (plaque) assays for the determination of viral titers, and the use of different ASFV-sensitive cells as targets for virus production and titration. Curr. Protoc. Cell Biol. 53:26.14.1-26.14.25. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • African swine fever virus (ASFV);
  • susceptibility;
  • production;
  • purification;
  • titration;
  • hemadsorption;
  • cytopathic effect;
  • plaque assay;
  • target cells;
  • Vero;
  • COS-1;
  • IPAM;
  • WSL;
  • swine macrophages