Miscellaneous
UNIT 26.14 Methods for Growing and Titrating African Swine Fever Virus: Field and Laboratory Samples
Published Online: 1 DEC 2011
DOI: 10.1002/0471143030.cb2614s53
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
Additional Information
How to Cite
Carrascosa, A. L., Bustos, M. J. and de Leon, P. 2011. Methods for Growing and Titrating African Swine Fever Virus: Field and Laboratory Samples. Current Protocols in Cell Biology. 53:26.14.1–26.14.25.
Publication History
- Published Online: 1 DEC 2011
- Published Print: DEC 2011
- Abstract
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Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and protocols to produce and titrate any ASFV strain in cell cultures. The procedures include (1) basic techniques to prepare virus-sensitive target cells; (2) strategies for growth, concentration, and purification of virus stocks; and (3) the semi-quantitative (end dilution) and quantitative (plaque) assays for the determination of viral titers, and the use of different ASFV-sensitive cells as targets for virus production and titration. Curr. Protoc. Cell Biol. 53:26.14.1-26.14.25. © 2011 by John Wiley & Sons, Inc.
Keywords: African swine fever virus (ASFV); susceptibility; production; purification; titration; hemadsorption; cytopathic effect; plaque assay; target cells; Vero; COS-1; IPAM; WSL; swine macrophages