Unit

UNIT 27.1 Silencing of Gene Expression in Cultured Cells Using Small Interfering RNAs

  1. Kumi Sakurai,
  2. Pritsana Chomchan,
  3. John J. Rossi

Published Online: 1 JUN 2010

DOI: 10.1002/0471143030.cb2701s47

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Sakurai, K., Chomchan, P. and Rossi, J. J. 2010. Silencing of Gene Expression in Cultured Cells Using Small Interfering RNAs. Current Protocols in Cell Biology. 47:27.1:27.1.1–27.1.28.

Author Information

  1. Beckman Research Institute of City of Hope, Duarte, California

Publication History

  1. Published Online: 1 JUN 2010
  2. Published Print: JUN 2010

Abstract

The discovery of RNA interference (RNAi) and related small RNA–mediated regulatory pathways has significantly altered the understanding of gene regulation in eukaryotic cells. In the RNAi pathway, small interfering RNAs (siRNAs) ∼21 to 23 nucleotides in length serve as the regulatory molecules that guide and induce sequence-specific gene silencing. The use of siRNA-mediated silencing as a tool for investigating gene function is well established in cultured mammalian cells. This unit provides basic approaches to explore the field of RNAi, and hopes to address the importance of optimizing transfection conditions after empirical determinations in order to understand various degrees of silencing efficiency. Curr. Protoc. Cell Biol. 47:27.1.1-27.1.28. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • RNAi;
  • small silencing RNAs;
  • gene silencing;
  • transfection;
  • mammalian cell culture