Appendix

APPENDIX 1G Centrifuges and Rotors

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cba01gs07

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

2001. Centrifuges and Rotors. Current Protocols in Cell Biology. 7:1G:A.1G.1–A.1G.7.

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

This appendix lists rmax values (maximum value of the rotating radius between the particle being centrifuged and the axis of rotation) for commonly used rotors manufactured by Sorvall, Beckman Coulter, Fisher, and IEC. In addition, nomograms are provided for the determination of the relative centrifugal force (RCF) where speed and rmax are known, or the speed where RCF and rmax are known. This is done by aligning a ruler across the two known values and reading the unknown value at the point where the ruler crosses the remaining column. Also discussed are the characteristics of the various materials used to construct centrifuge tubes, including optical properties, appropriate methods for sterilization, and chemical resistances (tolerance to various media, organic solvents, and alcohols).