APPENDIX 1G Centrifuges and Rotors
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
2001. Centrifuges and Rotors. Current Protocols in Cell Biology. 7:1G:A.1G.1–A.1G.7.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
This appendix lists rmax values (maximum value of the rotating radius between the particle being centrifuged and the axis of rotation) for commonly used rotors manufactured by Sorvall, Beckman Coulter, Fisher, and IEC. In addition, nomograms are provided for the determination of the relative centrifugal force (RCF) where speed and rmax are known, or the speed where RCF and rmax are known. This is done by aligning a ruler across the two known values and reading the unknown value at the point where the ruler crosses the remaining column. Also discussed are the characteristics of the various materials used to construct centrifuge tubes, including optical properties, appropriate methods for sterilization, and chemical resistances (tolerance to various media, organic solvents, and alcohols).