APPENDIX 3C Dialysis and Concentration of Protein Solutions
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Andrew, S. M., Titus, J. A. and Zumstein, L. 2001. Dialysis and Concentration of Protein Solutions. Current Protocols in Cell Biology. 4:3C:A.3C.1–A.3C.5.
- Published Online: 1 MAY 2001
- Published Print: OCT 1999
Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. Dialysis is usually used to change the salt (small-molecule) composition of a macromolecule-containing solution. This unit describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A support protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.