APPENDIX 3C Dialysis and Concentration of Protein Solutions

  1. Sarah. M. Andrew1,
  2. Julie. A. Titus2,
  3. Louis Zumstein3

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cba03cs04

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Andrew, S. M., Titus, J. A. and Zumstein, L. 2001. Dialysis and Concentration of Protein Solutions. Current Protocols in Cell Biology. 4:3C:A.3C.1–A.3C.5.

Author Information

  1. 1

    Chester College, Chester, United Kingdom

  2. 2

    National Cancer Institute, Bethesda, Maryland

  3. 3

    Introgen Therapeutics, Inc., Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999


Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. Dialysis is usually used to change the salt (small-molecule) composition of a macromolecule-containing solution. This unit describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A support protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.