Appendix

APPENDIX 3D Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy

  1. Sean R. Gallagher

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cba03ds07

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Gallagher, S. R. 2001. Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Cell Biology. 7:3D:A.3D.1–A.3D.8.

Author Information

  1. Motorola Corporation, Tempe, Arizona

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in cell and molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques–Hoescht 33258 dye binding, ethidium bromide binding are described. These three procedures cover a range from 5 to 10 ng/ml DNA to 50m g/ml DNA.