APPENDIX 3D Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Gallagher, S. R. 2001. Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Cell Biology. 7:3D:A.3D.1–A.3D.8.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in cell and molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques–Hoescht 33258 dye binding, ethidium bromide binding are described. These three procedures cover a range from 5 to 10 ng/ml DNA to 50m g/ml DNA.