APPENDIX 3F Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Kramer, M. F. and Coen, D. M. 2001. Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization. Current Protocols in Cell Biology. 10:3F:A.3F.1–A.3F.14.
- Published Online: 1 MAY 2001
- Published Print: APR 2001
This appendix describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequence and primer sets of interest, and to optimize for specificity, sensitivity, and yield.