Appendix

APPENDIX 3G Micro RT-PCR

  1. Cloud P. Paweletz,
  2. Lu Charboneau,
  3. Lance A. Liotta

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cba03gs10

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Paweletz, C. P., Charboneau, L. and Liotta, L. A. 2001. Micro RT-PCR. Current Protocols in Cell Biology. 10:3G:A.3G.1–A.3G.6.

Author Information

  1. National Cancer Institute/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

Abstract

This unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).