Appendix

APPENDIX 3H The Colorimetric Detection and Quantitation of Total Protein

  1. Randall I. Krohn

Published Online: 1 SEP 2011

DOI: 10.1002/0471143030.cba03hs52

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Krohn, R. I. 2011. The Colorimetric Detection and Quantitation of Total Protein. Current Protocols in Cell Biology. 52:3H:A.3H.1–A.3H.28.

Author Information

  1. Pierce Biotechnology Inc., Rockford, Illinois

Publication History

  1. Published Online: 1 SEP 2011
  2. Published Print: SEP 2011

Abstract

Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. Colorimetric methods are fast, simple, and not laborious. This unit describes a number of assays able to detect protein concentrations in the low microgram to milligram per milliliter ranges in a variety of formats. Curr. Protoc. Cell Biol. 52:A.3H.1-A.3H.28. © 2011 by John Wiley & Sons, Inc.