Chapter 10. DNA Sequencing: Current Tactics
Published Online: 20 JUN 2002
DOI: 10.1002/0471220566.ch10
Copyright © 1999 John Wiley & Sons, Inc.
Book Title
Genomics: The Science and Technology Behind the Human Genome Project
Additional Information
How to Cite
Cantor, C. R. and Smith, C. L. (2002) DNA Sequencing: Current Tactics, in Genomics: The Science and Technology Behind the Human Genome Project, John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471220566.ch10
Publication History
- Published Online: 20 JUN 2002
- Published Print: 2 FEB 1999
ISBN Information
Print ISBN: 9780471599081
Online ISBN: 9780471220565
- Summary
- Chapter
Keywords:
- DNA sequencing;
- ladder sequencing;
- fluorescent DNA sequencing;
- DNA sequencing chemistry;
- mass spectrometry;
- rate-limiting steps
Summary
From the rate of progress in our ability to interpret DNA sequences de novo in terms of plausible gene function, we can be reasonably optimistic that coding regions will be identifiable with almost perfect accuracy and that most new genes will carry in their sequence immediately recognizable clues about function. This chapter presents a discussion of current tactics in DNA sequencing.