Chapter 11. Strategies for Large-Scale DNA Sequencing
Published Online: 20 JUN 2002
DOI: 10.1002/0471220566.ch11
Copyright © 1999 John Wiley & Sons, Inc.
Book Title
Genomics: The Science and Technology Behind the Human Genome Project
Additional Information
How to Cite
Cantor, C. R. and Smith, C. L. (2002) Strategies for Large-Scale DNA Sequencing, in Genomics: The Science and Technology Behind the Human Genome Project, John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471220566.ch11
Publication History
- Published Online: 20 JUN 2002
- Published Print: 2 FEB 1999
ISBN Information
Print ISBN: 9780471599081
Online ISBN: 9780471220565
- Summary
- Chapter
Keywords:
- large-scale DNA sequencing;
- shotgun DNA sequencing;
- directed sequencing;
- primers;
- short oligonucleotides;
- transposon-mediated DNA sequencing;
- delta restriction cloning;
- nested deletions;
- primer jumping;
- primer multiplexing;
- multiplex genomic walking;
- uneven cDNA distribution;
- complete genome sequence;
- fifth base
Summary
There are several well-established, basic strategies for sequencing DNA targets on the scale of cosmids. The basic strategies range from shotgun, which is a purely random approach, to highly directed walking methods. In this chapter the principles behind these strategies are illustrated and some of their relative advantages and disadvantages are described. In addition the authors discuss the relative merits of sequencing genomes and sequencing just the genes, by the use of cDNA libraries. Current approaches for both of these objectives are described.