Chapter 12. Future DNA Sequencing without Length Fractionation
Published Online: 20 JUN 2002
DOI: 10.1002/0471220566.ch12
Copyright © 1999 John Wiley & Sons, Inc.
Book Title
Genomics: The Science and Technology Behind the Human Genome Project
Additional Information
How to Cite
Cantor, C. R. and Smith, C. L. (2002) Future DNA Sequencing without Length Fractionation, in Genomics: The Science and Technology Behind the Human Genome Project, John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471220566.ch12
Publication History
- Published Online: 20 JUN 2002
- Published Print: 2 FEB 1999
ISBN Information
Print ISBN: 9780471599081
Online ISBN: 9780471220565
- Summary
- Chapter
Keywords:
- DNA sequencing;
- length fractionation;
- single-molecule sequencing;
- high-resolution microscopy;
- stepwise enzymatic sequencing;
- sequencing by hybridization (SBH);
- branch point ambiguities;
- oligonucleotide chips;
- oligonucleotide stacking hybridization;
- positional sequencing by hybridization (PSBH)
Summary
There is considerable current interest in trying to develop entirely new approaches to DNA sequencing. Many of the techniques that are mentioned in this chapter are discussed only briefly. However, at least one of the second-generation methods treated in this chapter, sequencing by hybridization (SBH), does appear to offer a significant chance of making an impact on the current human genome project, and an even better chance of making a major impact on future DNA sequencing in clinical diagnostics.