Chapter 14. Sequence-Specific Manipulation of DNA

  1. Charles R. Cantor,
  2. Cassandra L. Smith

Published Online: 20 JUN 2002

DOI: 10.1002/0471220566.ch14

Book Title

Genomics: The Science and Technology Behind the Human Genome Project

Genomics: The Science and Technology Behind the Human Genome Project

Additional Information

How to Cite

Cantor, C. R. and Smith, C. L. (2002) Sequence-Specific Manipulation of DNA, in Genomics: The Science and Technology Behind the Human Genome Project, John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471220566.ch14

Author Information

  1. Center for Advanced Biotechnology, Boston University, Boston, Massachusetts

Publication History

  1. Published Online: 20 JUN 2002
  2. Published Print: 2 FEB 1999

ISBN Information

Print ISBN: 9780471599081

Online ISBN: 9780471220565

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CHAPTER TOOLS

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Keywords:

  • DNA manipulation;
  • base-base recognition;
  • specificity;
  • triple-stranded DNA;
  • triplex-mediated DNA cleavage;
  • sequence-specific DNA capture;
  • triplex-mediated DNA capture;
  • affinity capture electrophoresis;
  • backbone analogues;
  • sequence-specific cloning;
  • coincidence cloning;
  • repeated DNA sequences;
  • PCR;
  • repeat expansion detection;
  • aptamer selection;
  • oligonucleotides

Summary

In this chapter various methods are described that take advantage of the specific recognition of DNA sequences to allow analytical or preparative procedures to be carried out on a selected fraction of a complex DNA sample. For example, one can design chemical or enzymatic schemes to cut at extremely specific DNA sites, to purify specific DNA sequences, or to isolate selected classes of DNA sequences.

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