Chapter 3. Analysis of DNA Sequences by Hybridization
Published Online: 20 JUN 2002
DOI: 10.1002/0471220566.ch3
Copyright © 1999 John Wiley & Sons, Inc.
Book Title
Genomics: The Science and Technology Behind the Human Genome Project
Additional Information
How to Cite
Cantor, C. R. and Smith, C. L. (2002) Analysis of DNA Sequences by Hybridization, in Genomics: The Science and Technology Behind the Human Genome Project, John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471220566.ch3
Publication History
- Published Online: 20 JUN 2002
- Published Print: 2 FEB 1999
ISBN Information
Print ISBN: 9780471599081
Online ISBN: 9780471220565
- Summary
- Chapter
Keywords:
- DNA sequences;
- hybridization;
- selectivity;
- sensitivity;
- double strands;
- single strands;
- thermodynamics;
- melting;
- short duplexes;
- imperfectly paired duplexes;
- filters;
- sensitive detection
Summary
By current methodology we are able to determine the presence or absence of almost any 20-bp DNA sequence within a single cell. This analysis takes place at the level of single molecules. Two requirements must be met to perform such an exquisitely demanding analysis. There must be sufficient experimental sensitivity to detect the presence of the sequence. There must also be sufficient experimental selectivity to discriminate between the desired, true target sequence and all other similar sequences, which may differ from the target by as little as one base. This chapter presents a discussion of the analysis of DNA sequences by hybridization.