Chapter 5. Principles of DNA Electrophoresis
Published Online: 20 JUN 2002
DOI: 10.1002/0471220566.ch5
Copyright © 1999 John Wiley & Sons, Inc.
Book Title
Genomics: The Science and Technology Behind the Human Genome Project
Additional Information
How to Cite
Cantor, C. R. and Smith, C. L. (2002) Principles of DNA Electrophoresis, in Genomics: The Science and Technology Behind the Human Genome Project, John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471220566.ch5
Publication History
- Published Online: 20 JUN 2002
- Published Print: 2 FEB 1999
ISBN Information
Print ISBN: 9780471599081
Online ISBN: 9780471220565
- Summary
- Chapter
Keywords:
- DNA electrophoresis;
- fractionation separation;
- ultracentrifuge;
- size separations;
- gel structure;
- gel behavior;
- reptation models;
- pulsed field gel electrophoresis (PFG);
- DNA trapping electrophoresis;
- secondary pulsed field gel electrophoresis (SPFG)
Summary
In this chapter the authors briefly review DNA fractionations other than electrophoresis. The bulk of the chapter is spent on trying to integrate the maze of experimental and modeling observations that constitute our present-day knowledge of the principles that underlie DNA electrophoresis. Practical aspects of DNA separations are presented.