Chapter 2. Cell Interaction and Epithelial Differentiation

  1. R. Ian Freshney and
  2. Mary G. Freshney
  1. Nicole Maas-Szabowski,
  2. Hans-Jürgen Stark and
  3. Norbert E. Fusenig

Published Online: 28 APR 2002

DOI: 10.1002/0471221201.ch2

Culture of Epithelial Cells, Second Edition

Culture of Epithelial Cells, Second Edition

How to Cite

Maas-Szabowski, N., Stark, H.-J. and Fusenig, N. E. (2002) Cell Interaction and Epithelial Differentiation, in Culture of Epithelial Cells, Second Edition (eds R. I. Freshney and M. G. Freshney), John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471221201.ch2

Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

Author Information

  1. Division of Carcinogenesis and Differentiation, German Cancer Research Center (Deutsches Krebsforschungszentrum), Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany

Publication History

  1. Published Online: 28 APR 2002
  2. Published Print: 12 APR 2002

Book Series:

  1. Culture of Specialized Cells

Book Series Editors:

  1. R. Ian Freshney

Series Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

ISBN Information

Print ISBN: 9780471401216

Online ISBN: 9780471221203

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Keywords:

  • epidermal keratinocytes;
  • epithelial cell culture;
  • cell-cell interaction;
  • KGF;
  • GM-CSF;
  • c-jun;
  • junB;
  • transplantation;
  • dermal equivalent;
  • cell differentiation;
  • cell proliferation;
  • organotypic culture;
  • paracrine factors;
  • dermal fibroblasts;
  • IL-1;
  • collagen matrix

Summary

This chapter focuses on the role of dermal fibroblasts in epidermal keratinocyte proliferation and differentiation and proves the usefulness of an organotypic in vitro skin equivalent model to study mechanisms of tissue regeneration. Examples are given how these can be used to study the growth of keratinocytes after transplantation in vivo, alone or with a collagen matrix. This leads on to the development of in vitro organotypic models to generate skin equivalents composed of an organized epidermis on matrix-embedded dermal fibroblasts. Protocols are provided for both the in vivo transplantation models and the combined stromal/epithelial in vitro organotypic models, along with several variations of the methods. Characterization of the proliferation and differentiation of the cultures is described with an extensive discussion of recent results on the factors controlling tissue regeneration, with emphasis on the role of c-jun and junB gene products in the production of KGF and GM-CSF by fibroblasts in response to epithelium-derived IL1, and the effect of these cytokines on the proliferation and differentiation of epidermal keratinocytes.