Chapter 3. The Epidermis

  1. R. Ian Freshney and
  2. Mary G. Freshney
  1. E. Kenneth Parkinson1 and
  2. W. Andrew Yeudall2

Published Online: 28 APR 2002

DOI: 10.1002/0471221201.ch3

Culture of Epithelial Cells, Second Edition

Culture of Epithelial Cells, Second Edition

How to Cite

Parkinson, E. K. and Yeudall, W. A. (2002) The Epidermis, in Culture of Epithelial Cells, Second Edition (eds R. I. Freshney and M. G. Freshney), John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471221201.ch3

Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

Author Information

  1. 1

    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK

  2. 2

    Molecular Carcinogenesis Group, Guy's King's & St.Thomas' Schools of Medicine & Dentistry, King's College London, London SE1 9RT, UK

Publication History

  1. Published Online: 28 APR 2002
  2. Published Print: 12 APR 2002

Book Series:

  1. Culture of Specialized Cells

Book Series Editors:

  1. R. Ian Freshney

Series Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

ISBN Information

Print ISBN: 9780471401216

Online ISBN: 9780471221203

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Keywords:

  • epidermal keratinocytes;
  • epithelial cell culture;
  • trypsin disaggregation;
  • serum-free media;
  • MCDB 153;
  • 3T3 feeder layers;
  • cell differentiation;
  • squamous cell carcinoma;
  • cryopreservation

Summary

Various attempts at growing keratinocytes are given in an historical review, with emphasis on the 3T3 feeder layer technique. Protocols are provided for the disaggregation of skin by warm or cold trypsin, and the subsequent culture of epidermal keratinocytes on 3T3 feeder layers. Subculture and the removal of fibroblasts are described, and details given for cryopreservation. The relative merits of culture in selective serum-free media are compared with culture on 3T3 feeder cells. Culture methods for cells derived from keratinocyte tumors are discussed and their resistance to terminal differentiation noted. The chapter concludes with a section on epidermal keratinocyte markers and presents optimal conditions for their induction.