Chapter 6. Human Prostatic Epithelial Cells

  1. R. Ian Freshney and
  2. Mary G. Freshney
  1. Donna M. Peehl

Published Online: 28 APR 2002

DOI: 10.1002/0471221201.ch6

Culture of Epithelial Cells, Second Edition

Culture of Epithelial Cells, Second Edition

How to Cite

Peehl, D. M. (2002) Human Prostatic Epithelial Cells, in Culture of Epithelial Cells, Second Edition (eds R. I. Freshney and M. G. Freshney), John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471221201.ch6

Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

Author Information

  1. Department of Urology, Stanford University School of Medicine, Stanford, CA 94305, USA

Publication History

  1. Published Online: 28 APR 2002
  2. Published Print: 12 APR 2002

Book Series:

  1. Culture of Specialized Cells

Book Series Editors:

  1. R. Ian Freshney

Series Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

ISBN Information

Print ISBN: 9780471401216

Online ISBN: 9780471221203

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Keywords:

  • prostatic epithelium;
  • cell culture;
  • collagenase disaggregation;
  • serum-free medium;
  • cytokeratins;
  • cryopreservation

Summary

Chapter 6 gives a short introduction to the biology of the prostate and the importance of benign hyperplasia and neoplasia. The methodology covers the disaggregation of human prostate in collagenase and subsequent primary culture of the epithelial cell component, enriched by brief differential centrifugation of the partially disaggregated clumps from single cell dispersed stroma, in a specifically designed selective serum-free medium. Methods for cryopreservation are also described and variations used by other authors are discussed as are continuous cell lines derived from prostatic tumors. Methods of identification include morphology, cytokeratins, and the expression of tissue specific markers such as PSA, but expression in vitro requires matrix or stromal cell interaction. The role of cell differentiation on marker expression is also discussed.