Chapter 8. Normal Human Bronchial Epithelial Cell Culture

  1. R. Ian Freshney and
  2. Mary G. Freshney
  1. John Wise1 and
  2. John F. Lechner2

Published Online: 28 APR 2002

DOI: 10.1002/0471221201.ch8

Culture of Epithelial Cells, Second Edition

Culture of Epithelial Cells, Second Edition

How to Cite

Wise, J. and Lechner, J. F. (2002) Normal Human Bronchial Epithelial Cell Culture, in Culture of Epithelial Cells, Second Edition (eds R. I. Freshney and M. G. Freshney), John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471221201.ch8

Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

Author Information

  1. 1

    Yale University, School of Medicine, New Haven, CT 06520, USA

  2. 2

    Bayer Diagnostics, Emeryville, CA 94608, USA

Publication History

  1. Published Online: 28 APR 2002
  2. Published Print: 12 APR 2002

Book Series:

  1. Culture of Specialized Cells

Book Series Editors:

  1. R. Ian Freshney

Series Editor Information

  1. CRC Beatson Laboratories, Glasgow, Scotland

ISBN Information

Print ISBN: 9780471401216

Online ISBN: 9780471221203

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Keywords:

  • bronchial epithelium;
  • epithelial cell culture;
  • primary explants;
  • ischemia;
  • NHBE;
  • serum-free medium;
  • LHC-9;
  • matrix coating;
  • PAS;
  • cytokeratin;
  • cryopreservation

Summary

Hyperplasia, squamous metaplasia, and dysplasia are common reactions of human airway epithelia to chemical and physical cytotoxic agents. These processes demand strict interregulation of cell replication and squamous and mucoepidermoid differentiation control mechanisms. Aberrations in these processes positively correlate with a high risk for neoplastic transformation. Replicative cultures of normal human bronchial epithelial (NHBE) cells offer opportunities to study these phenomena. This chapter reviews methodologies commonly used to establish these cultures and briefly describes potential modifications. A protocol is provided for reversing ischemic damage in explanted fragments which are then used to create outgrowth of NHBE cells in serum-free medium on a matrix coated surface. NHBE cells may be identified by prekeratin and keratin expression and alcian blue-PAS staining, and both cells and explants can be cryopreserved in a DMSO-containing medium.