Chapter 12. Elements of DNA Vaccine Design
- Peter J. Quesenberry2,
- Gary S. Stein2,
- Bernard G. Forget3,
- Sherman M. Weissman3
Published Online: 13 MAY 2002
DOI: 10.1002/0471223956.ch12
Copyright © 1998 by Wiley-Liss, Inc.
Book Title
Stem Cell Biology and Gene Therapy
Additional Information
How to Cite
Caulfield, M. J. and Liu, M. A. (2002) Elements of DNA Vaccine Design, in Stem Cell Biology and Gene Therapy (eds P. J. Quesenberry, G. S. Stein, B. G. Forget and S. M. Weissman), John Wiley & Sons, Inc., New York, USA. doi: 10.1002/0471223956.ch12
Editor Information
- 2
University of Massachusetts, Worcester, Massachusetts
- 3
Yale University School of Medicine, New Haven, Connecticut
Publication History
- Published Online: 13 MAY 2002
- Published Print: 27 AUG 1998
ISBN Information
Print ISBN: 9780471146568
Online ISBN: 9780471223955
- Summary
- Chapter
Keywords:
- DNA vaccine;
- delivery;
- gene expression;
- protein expression;
- antigen processing;
- cell-mediated responses;
- humoral responses;
- tolerance;
- immunity
Summary
This review is intended to outline the elements of DNA vaccine design as they relate to the induction of immunity with the purpose of identifying areas for future research. Although the basic design of plasmid vaccines appears to be set to include a viral promoter (usually CMV), the gene of interest, and a terminator (often from BGH), there may be room to improve the various elements of a DNA vaccine to enhance expression and immunogenicity. The search for better promoters and enhancers will continue, and the observation that sequences from the target gene may influence expression and immunogenicity (as is the case with OspA) indicates the potential need to match target genes with compatible promoters and other regulatory elements.