Chapter 1. Alternative Strategies for Targeted Delivery of Nucleic Acid–Liposome Complexes

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Nancy Smyth Templeton Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch1

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Templeton, N. S. (2003) Alternative Strategies for Targeted Delivery of Nucleic Acid–Liposome Complexes, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch1

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Center for Cell and Gene Therapy, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

SEARCH

Keywords:

  • non-viral delivery;
  • gene therapy;
  • targeting;
  • gene delivery;
  • cell entry;
  • bilamellar invaginated vesicles (BIV);
  • liposomes;
  • extravasation;
  • systemic therapeutics;
  • non-immunogenic

Summary

Liposomes provide efficient delivery of therapeutic agents that can be repeatedly administered, are non-immunogenic, can penetrate beyond tight barriers to reach disease targets, and can be regulated as drugs versus biologics. Furthermore, using improved technologies, liposomes can be targeted for delivery to specific cell surface receptors resulting in efficient nuclear uptake of nucleic acids within the cell. Current methods have been inefficient in producing high levels of gene expression in target cells after delivery of plasmid DNA. This chapter focuses on the problems of the current targeting strategies and on new approaches that overcome the obstacles discussed.