Chapter 17. Generation of Safe, Targetable Sindbis Vectors That Have the Potential for Direct in vivo Gene Therapy

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Daniel Meruelo Ph.D.,
  2. Brandi Levin M.A. and
  3. Christine Pampeno Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch17

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Meruelo, D., Levin, B. and Pampeno, C. (2003) Generation of Safe, Targetable Sindbis Vectors That Have the Potential for Direct in vivo Gene Therapy, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch17

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, New York, New York, USA

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • Sindbis vectors;
  • in vivo gene therapy;
  • retargeting alphaviruses;
  • mosquito cells;
  • packaging cell lines;
  • targetable Sindbis vectors;
  • results;
  • undiminished infectious titers

Summary

Gene therapy for various inherited and acquired disorders would benefit greatly from the availability of a vector that has a high efficiency of gene expression and the ability to target cells in vivo. Our particular interest is cancer; therefore, although the vectors described in this chapter have potentially broad applicability, the focus of our discussion will be their utility for cancer therapy. A major goal of our research efforts has been to expand the utility of Sindbis vectors by rendering them directly targetable in vivo. This chapter describes the status of our efforts to date in generating such vectors.