Chapter 20. Targeting Bacteriophage Vectors

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Isabella Saggio Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch20

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Saggio, I. (2003) Targeting Bacteriophage Vectors, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch20

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Department of Genetics and Molecular Biology, University of Rome “La Sapienza” and Parco Scientifico Biomedico di Roma, S. Raffaele, Italy

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • phage;
  • lambda;
  • penton base;
  • adenovirus;
  • endocytosis;
  • bacteriophage;
  • integrin;
  • targeting

Summary

Viruses represent the most efficient system to encapsidate, protect and deliver DNA into cells. This fact has stimulated their exploitment as gene transfer vectors for mammalian cells. Nevertheless, their physical complexity has frequently represented an obstacle for the production of targeted constructs. In addition, eukaryotic safety problems have been encountered when analyzing in vivo these virus-based preparations. In this chapter we will present innovative delivery systems, based on prokaryotic viruses, engineered to target eukaryotic receptors. These chimera should ensure several theoretical advantages: low costs, reduced toxicity, and high selectivity. The specific components of these chimera and their properties, analyzed in a gene transfer perspective, will be detailed in the different sections of the chapter.