Chapter 24. Clostridium-Mediated Transfer of Therapeutic Proteins to Solid Tumors
- David T. Curiel M.D.,
- Joanne T. Douglas Ph.D.
Published Online: 31 MAR 2003
Copyright © 2002 John Wiley & Sons, Inc.
Vector Targeting for Therapeutic Gene Delivery
How to Cite
Lambin, P., Theys, J., Nuyts, S., Landuyt, W., Van Mellaert, L. and Anné, J. (2003) Clostridium-Mediated Transfer of Therapeutic Proteins to Solid Tumors, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch24
Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA
- Published Online: 31 MAR 2003
- Published Print: 9 AUG 2002
Print ISBN: 9780471434795
Online ISBN: 9780471234302
- gene transfer;
- anti-cancer treatment;
The presence of severe hypoxia and necrosis in solid tumors limits the efficacy of conventional treatment modalities. On the other hand, this unique physiology offers the potential to apply an anaerobic bacterial transfer system in cancer treatment. Systemically administered spores of non-pathogenic Clostridium bacteria specifically germinate into metabolically active cells in the hypoxic/necrotic regions of tumors. These clostridial cells can be genetically engineered to express and secrete therapeutic proteins such as cytosine deaminase or tumor necrosis factor a. The use of this alternative and innovative approach offers several advantages over classical gene therapy systems, including its high tumor-specificity. Selectivity can even be improved by the use of radio-inducible promoters offering spatial and increased temporal control of gene expression. Taken together, the data described here provide evidence for the potential application of Clostridium-based therapeutic protein transfer to tumors in anti-cancer therapy.