Chapter 25. Selection of Peptides on Phage
- David T. Curiel M.D.,
- Joanne T. Douglas Ph.D.
Published Online: 31 MAR 2003
Copyright © 2002 John Wiley & Sons, Inc.
Vector Targeting for Therapeutic Gene Delivery
How to Cite
Barry, M. A., Takahashi, S. and Parrott, M. B. (2003) Selection of Peptides on Phage, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch25
Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA
- Published Online: 31 MAR 2003
- Published Print: 9 AUG 2002
Print ISBN: 9780471434795
Online ISBN: 9780471234302
- phage display;
- peptide libraries;
- ligand translation;
- context-specific ligand function
Peptide-presenting phage libraries are a robust are a robust technology originally applied for the selection of peptides against single proteins, but that is now being applied for cell-targeting technologies. From recent work, it is clear that this technology can readily identify cell-binding peptides, but isolation of cell-specific peptides in most cases requires robust methods to focus selection on receptors unique to the target cell. The next technological hurdle for applying this technology will be developing approaches to retain both ligand and vector function when translating phage-identified peptides into the heterologous structural context of a gene delivery vector.