Chapter 25. Selection of Peptides on Phage

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Michael A. Barry Ph.D.1,
  2. Satoshi Takahashi M.D.2 and
  3. M. Brandon Parrott B.S.3

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch25

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Barry, M. A., Takahashi, S. and Parrott, M. B. (2003) Selection of Peptides on Phage, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch25

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. 1

    Center for Cell and Gene Therapy, Department of Molecular and Human Genetics, Baylor College of Medicine, and Department of Bioengineering, Rice University, Houston, Texas, USA

  2. 2

    Center for Gene Therapy, Baylor College of Medicine, Houston, Texas, USA

  3. 3

    Department of Immunology, Baylor College of Medicine, Houston, Texas, USA

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • phage display;
  • peptide libraries;
  • ligand translation;
  • context-specific ligand function

Summary

Peptide-presenting phage libraries are a robust are a robust technology originally applied for the selection of peptides against single proteins, but that is now being applied for cell-targeting technologies. From recent work, it is clear that this technology can readily identify cell-binding peptides, but isolation of cell-specific peptides in most cases requires robust methods to focus selection on receptors unique to the target cell. The next technological hurdle for applying this technology will be developing approaches to retain both ligand and vector function when translating phage-identified peptides into the heterologous structural context of a gene delivery vector.