Chapter 26. Antibody Phage Display Libraries for Use in Therapeutic Gene Targeting

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. P. Rohrbach Ph.D. and
  2. S. Dübel Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch26

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Rohrbach, P. and Dübel, S. (2002) Antibody Phage Display Libraries for Use in Therapeutic Gene Targeting, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch26

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Institut für Molekulare Genetik, Universität Heidelberg, Germany

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • recombinant antibody fragments;
  • combinatorial libraries;
  • phage display;
  • single-chain fragment;
  • panning;
  • antigen

Summary

Both production and panning of recombinant antibody fragments from antibody libraries have evolved to become one of the major sources for novel antibodies. The key technological development in that process was the use of a selection system showing efficiencies comparable to our own immune system. By displaying antibodies on the surface of microorganisms containing the genetic information of the antibody's gene within its genome, clones expressing antigen specific binding can easily be amplified. Antibody libraries consisting of more than 10 billion different members allow for the isolation of human antibody fragments against almost every possible antigen.