Chapter 27. Single-Chain Fv Fragments from Phage Display Libraries

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Roland E. Kontermann Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch27

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Kontermann, R. E. (2002) Single-Chain Fv Fragments from Phage Display Libraries, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch27

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Vectron Therapeutics AG, Marburg, Germany

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • single-chain Fv;
  • phage display;
  • biopanning;
  • cell selection;
  • in vivo selection;
  • vector targeting;
  • viral vectors;
  • non-viral vectors

Summary

The combination of single-chain Fv fragments as target cell-binding molecules with the phage display technology has led to powerful strategies to obtain scFv molecules applicable for vector targeting in gene therapy. This chapter describes the various methods which have been employed to isolate scFv molecules with the desired binding characteristics and strategies to use these molecules to generate targeted gene therapeutic vehicles.