Chapter 28. Retroviral Particle Display for Complex Glycosylated and Disulfide-Bonded Protein Domains

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Samuel C. Kayman Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch28

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Kayman, S. C. (2002) Retroviral Particle Display for Complex Glycosylated and Disulfide-Bonded Protein Domains, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch28

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Laboratory of Retroviral Biology, Public Health Research Institute, New York, New York, USA

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • particle display;
  • surface display;
  • retrovirus;
  • murine leukemia virus;
  • library screening;
  • glycosylation;
  • enrichment

Summary

Surface display systems allow enrichment of polypeptides with specific binding properties from libraries of sequence variants. A retroviral particle display system is described in which complex protein domains are inserted into the hypervariable region of a murine leukemia virus surface protein. Inserted domains are glycosylated, form multiple correct disulfide bonds, and are displayed on infectious virions. Enrichment of particles with specific binding properties due to inserted domains has been demonstrated, although recovery of bound virions requires further optimization. This system may extend display methodology to complex structures with binding properties that depend on post-translational modifications performed only by mammalian cells.