Chapter 28. Cell Surface Display and Cytometric Screening for Protein Ligand Isolation and Engineering
- David T. Curiel M.D. and
- Joanne T. Douglas Ph.D.
Published Online: 31 MAR 2003
Copyright © 2002 John Wiley & Sons, Inc.
Vector Targeting for Therapeutic Gene Delivery
How to Cite
Daugherty, P. S. (2003) Cell Surface Display and Cytometric Screening for Protein Ligand Isolation and Engineering, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch29
Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA
- Published Online: 31 MAR 2003
- Published Print: 9 AUG 2002
Print ISBN: 9780471434795
Online ISBN: 9780471234302
- cell-surface display;
- flow cytometry;
- ligand isolation;
- affinity maturation;
- high-throughput screening
Display technologies for protein engineering offer the potential to address various problems in gene targeting. In particular, protein display on the surface of bacteria and yeast coupled with quantitative high-throughput screening using flow cytometry has emerged as a powerful method to select ligands for particular receptors, and improve their binding affinity, specificity, and expression characteristics. Despite the proven usefulness of the widely used bacteriophage display method, cell surface display and cytometric screening offers an expanded range of applications allowing more precise affinity engineering and the display of complex proteins likely to be important for cellular targeting.