Chapter 28. Cell Surface Display and Cytometric Screening for Protein Ligand Isolation and Engineering

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Patrick S. Daugherty Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch29

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Daugherty, P. S. (2003) Cell Surface Display and Cytometric Screening for Protein Ligand Isolation and Engineering, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch29

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. Department of Chemical Engineering, University of California at Santa Barbara, Santa Barbara, California, USA

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • cell-surface display;
  • flow cytometry;
  • ligand isolation;
  • affinity maturation;
  • high-throughput screening

Summary

Display technologies for protein engineering offer the potential to address various problems in gene targeting. In particular, protein display on the surface of bacteria and yeast coupled with quantitative high-throughput screening using flow cytometry has emerged as a powerful method to select ligands for particular receptors, and improve their binding affinity, specificity, and expression characteristics. Despite the proven usefulness of the widely used bacteriophage display method, cell surface display and cytometric screening offers an expanded range of applications allowing more precise affinity engineering and the display of complex proteins likely to be important for cellular targeting.