Chapter 30. Monitoring Gene Therapy by Positron Emission Tomography
- David T. Curiel M.D.,
- Joanne T. Douglas Ph.D.
Published Online: 31 MAR 2003
DOI: 10.1002/0471234303.ch30
Copyright © 2002 John Wiley & Sons, Inc.
Book Title
Vector Targeting for Therapeutic Gene Delivery
Additional Information
How to Cite
Herschman, H. R., Barrio, J. R., Satyamurthy, N., Liang, Q., Maclaren, D. C., Yaghoubi, S., Toyokuni, T., Cherry, S. R., Phelps, M. E. and Gambhir, S. S. (2003) Monitoring Gene Therapy by Positron Emission Tomography, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch30
Editor Information
Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA
Publication History
- Published Online: 31 MAR 2003
- Published Print: 9 AUG 2002
ISBN Information
Print ISBN: 9780471434795
Online ISBN: 9780471234302
- Summary
- Chapter
Keywords:
- positron emission tomography;
- dopamine receptor;
- herpes simplex virus thymidine kinase;
- reporter gene;
- PET scanning;
- adenovirus;
- spiperone;
- acycloguanosine;
- ganciclovir;
- penciclovir
Summary
Gene therapists cannot track location, magnitude or duration of gene expression following somatic gene delivery. Consequently, they cannot correlate somatic gene transfer and phenotypic responses, since variations in gene delivery, gene expression levels and duration of gene expression cannot be monitored following gene administration. We developed two reporter genes whose expression can be non-invasively, quantitatively and repetitively monitored, in living individuals, by reporter protein-dependent sequestration of positron-labeled reporter probes, using positron emission tomography. We describe gene delivery systems from which therapeutic and PET reporter genes are co-expressed and demonstrated, using microPET in murine models, their ability to quantitate somatic gene transfer.