Chapter 30. Monitoring Gene Therapy by Positron Emission Tomography

  1. David T. Curiel M.D. and
  2. Joanne T. Douglas Ph.D.
  1. Harvey R. Herschman Ph.D,
  2. Jorge R. Barrio Ph.D.,
  3. Nagichettiar Satyamurthy Ph.D.,
  4. Qianwa Liang Ph.D.,
  5. Duncan C. Maclaren Ph.D.,
  6. Shariar Yaghoubi B.S.,
  7. Tatsushi Toyokuni Ph.D.,
  8. Simon R. Cherry Ph.D.,
  9. Michael E. Phelps Ph.D. and
  10. Sanjiv S. Gambhir M.D., Ph.D.

Published Online: 31 MAR 2003

DOI: 10.1002/0471234303.ch30

Vector Targeting for Therapeutic Gene Delivery

Vector Targeting for Therapeutic Gene Delivery

How to Cite

Herschman, H. R., Barrio, J. R., Satyamurthy, N., Liang, Q., Maclaren, D. C., Yaghoubi, S., Toyokuni, T., Cherry, S. R., Phelps, M. E. and Gambhir, S. S. (2003) Monitoring Gene Therapy by Positron Emission Tomography, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch30

Editor Information

  1. Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA

Author Information

  1. UCLA School of Medicine, Los Angeles, California, USA

Publication History

  1. Published Online: 31 MAR 2003
  2. Published Print: 9 AUG 2002

ISBN Information

Print ISBN: 9780471434795

Online ISBN: 9780471234302

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Keywords:

  • positron emission tomography;
  • dopamine receptor;
  • herpes simplex virus thymidine kinase;
  • reporter gene;
  • PET scanning;
  • adenovirus;
  • spiperone;
  • acycloguanosine;
  • ganciclovir;
  • penciclovir

Summary

Gene therapists cannot track location, magnitude or duration of gene expression following somatic gene delivery. Consequently, they cannot correlate somatic gene transfer and phenotypic responses, since variations in gene delivery, gene expression levels and duration of gene expression cannot be monitored following gene administration. We developed two reporter genes whose expression can be non-invasively, quantitatively and repetitively monitored, in living individuals, by reporter protein-dependent sequestration of positron-labeled reporter probes, using positron emission tomography. We describe gene delivery systems from which therapeutic and PET reporter genes are co-expressed and demonstrated, using microPET in murine models, their ability to quantitate somatic gene transfer.