Chapter 4. Receptor-Directed Gene Delivery Using Molecular Conjugates
- David T. Curiel M.D.,
- Joanne T. Douglas Ph.D.
Published Online: 31 MAR 2003
DOI: 10.1002/0471234303.ch4
Copyright © 2002 John Wiley & Sons, Inc.
Book Title
Vector Targeting for Therapeutic Gene Delivery
Additional Information
How to Cite
Ziady, A. G. and Davis, P. B. (2003) Receptor-Directed Gene Delivery Using Molecular Conjugates, in Vector Targeting for Therapeutic Gene Delivery (eds D. T. Curiel and J. T. Douglas), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471234303.ch4
Editor Information
Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, The University of Alabama at Birmingham, USA
Publication History
- Published Online: 31 MAR 2003
- Published Print: 9 AUG 2002
ISBN Information
Print ISBN: 9780471434795
Online ISBN: 9780471234302
- Summary
- Chapter
Keywords:
- poly-L-lysine;
- polyethyleneimine;
- receptor-directed gene transfer;
- endosomal escape;
- DNA compaction;
- asialoglycoprotein receptor;
- serpin enzyme complex receptor;
- polymeric immunoglobulin receptor;
- transferrin receptor;
- epidermal growth factor receptor;
- fibroblast growth factor receptor
Summary
In an effort to achieve delivery of therapeutic genes specifically to particular target cells, using a non-viral vector, molecular conjugates consisting of a receptor ligand, polycation to bind to DNA, and plasmid DNA have been constructed. Some successful complexes have capitalized on DNA compaction, which protects the plasmid from nuclease degradation and facilitates nuclear access in non-dividing cells. The chemical construction of the complex, including the density of the ligands, the nature of the polycation, the stabilizing agents used, as well as the specific receptor targeted and the ligands selected all affect the success of the gene transfer.