Unit

UNIT 1.21 Access Guide to Human Proteinpedia

  1. Babylakshmi Muthusamy1,2,
  2. Joji Kurian Thomas1,3,
  3. T.S. Keshava Prasad1,2,3,
  4. Akhilesh Pandey1,4

Published Online: 1 MAR 2013

DOI: 10.1002/0471250953.bi0121s41

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Muthusamy, B., Thomas, J. K., Prasad, T. K. and Pandey, A. 2013. Access Guide to Human Proteinpedia. Current Protocols in Bioinformatics. 41:1.21:1.21.1–1.21.15.

Author Information

  1. 1

    Institute of Bioinformatics, Bangalore, India

  2. 2

    Centre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, India

  3. 3

    Amrita School of Biotechnology, Amrita University, Kollam, India

  4. 4

    McKusick-Nathans Institute of Genetic Medicine and Departments of Biological Chemistry, Pathology, and Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland

Publication History

  1. Published Online: 1 MAR 2013

Abstract

Human Proteinpedia (http://www.humanproteinpedia.org) is a publicly available proteome repository for sharing human protein data derived from multiple experimental platforms. It incorporates diverse features of the human proteome including protein-protein interactions, enzyme-substrate relationships, PTMs, subcellular localization, and expression of proteins in various human tissues and cell lines in diverse biological conditions including diseases. Through a publicly distributed annotation system developed especially for proteomic data, investigators across the globe can upload, view, and edit proteomic data even before they are published. Inclusion of information on investigators and laboratories that generated the data, as well as visualization of tandem mass spectra, stained tissue sections, protein/peptide microarrays, fluorescent micrographs, and western blots, ensures quality of proteomic data assimilated in Human Proteinpedia. Many of the protein annotations submitted to Human Proteinpedia have also been made available to the scientific community through Human Protein Reference Database (http://www.hprd.org), another resource developed by our group. In this protocol, we describe how to submit, edit, and retrieve proteomic data in Human Proteinpedia. Curr. Protoc. Bioinform. 41:1.21.1-1.21.15. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • mass spectrometry;
  • tissue microarrays;
  • biomarkers;
  • disease proteomics;
  • HPRD;
  • proteotypic peptides;
  • multiple reaction monitoring