UNIT 2.2 Using the Blocks Database to Recognize Functional Domains
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Henikoff, J. G., Greene, E. A., Taylor, N., Henikoff, S. and Pietrokovski, S. 2002. Using the Blocks Database to Recognize Functional Domains. Current Protocols in Bioinformatics. 00:2.2:2.2.1–2.2.32.
- Published Online: 1 AUG 2002
- Published Print: JAN 2003
Blocks are ungapped multiple alignments of related protein sequence segments that correspond to the most conserved regions of the proteins. The Blocks Database is a collection of blocks representing known protein families that can be used to compare a protein or DNA sequence with documented families of proteins. Protocols in this unit describe the analysis of proteins and families using Blocks-based tools, including searching, exploring relationships with trees, making new blocks, and designing PCR primers from blocks for isolating homologous sequences.