UNIT 2.10 Using the Structure-Function Linkage Database to Characterize Functional Domains in Enzymes
Published Online: 1 MAR 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Brown, S. and Babbitt, P. 2006. Using the Structure-Function Linkage Database to Characterize Functional Domains in Enzymes. Current Protocols in Bioinformatics. 13:2.10:2.10.1–2.10.16.
- Published Online: 1 MAR 2006
- Published Print: FEB 2006
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The Structure-Function Linkage Database (SFLD; http://sfld.rbvi.ucsf.edu/) is a Web-accessible database designed to link enzyme sequence, structure, and functional information. This unit describes the protocols by which a user may query the database to predict the function of newly sequenced enzymes and to correct misannotated functional assignments for enzymes currently in public databases. It is especially useful in helping a user discriminate functional capabilities of a sequence that is only distantly related to characterized sequences in publicly available databases.
- superfamily analysis;
- sequence analysis;
- structure-function relationships;
- function prediction;
- annotation transfer