UNIT 3.4 Finding Homologs in Amino Acid Sequences Using Network BLAST Searches
Published Online: 1 FEB 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Ladunga, I. 2003. Finding Homologs in Amino Acid Sequences Using Network BLAST Searches. Current Protocols in Bioinformatics. 00:3.4:3.4.1–3.4.33.
- Published Online: 1 FEB 2003
- Published Print: JAN 2003
This is not the most recent version of the article. View current version (1 MAR 2009)
BLAST, Basic Local Alignment Search Tool is used more frequently than any other biosequence database search program. The purpose of this unit is not only to show how to run searches on the Web, but also to demonstrate how to fine-tune arguments for a specific research project. It also offers guidance for interpreting results, handling statistical significance and biological relevance issues, and selecting complementary analyses. This unit covers three classes of the BLAST program: standard protein-to-protein searches, translated searches when either the query or the database consists of nucleotide sequences translated into proteins, and finally programs for comparing two sequences (as opposed to searching one sequence against a database of sequences).