UNIT 3.9 Finding Protein and Nucleotide Similarities with FASTA
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Pearson, W. 2004. Finding Protein and Nucleotide Similarities with FASTA. Current Protocols in Bioinformatics. 8:3.9:3.9.1–3.9.23.
- Published Online: 1 FEB 2004
- Published Print: DEC 2003
This is not the most recent version of the article. View current version (24 MAR 2016)
The FASTA package provides a comprehensive set of similarity searching programs, similar to those provided by the BLAST package, and some additional programs that are not provided by BLAST for searching with short peptides and oligonucleotides. The FASTA programs work with a wide variety of database formats, including mySQL sequence databases. FASTA provides very accurate statistical significance estimates, and is more sensitive than BLASTN when comparing DNA sequences. These protocols describe how to use the FASTA programs to characterize protein and DNA sequences, using protein:protein, protein:DNA, and DNA:DNA comparisons.