Unit

UNIT 3.13 Clustal Omega

  1. Fabian Sievers,
  2. Desmond G. Higgins

Published Online: 12 DEC 2014

DOI: 10.1002/0471250953.bi0313s48

Lab Protocol Title

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

Additional Information

How to Cite

Sievers, F., Higgins, D. G. 2014. Clustal Omega. Curr. Protoc. Bioinform. 48:1.25.1-1.25.33. doi: 10.1002/0471250953.bi0313s48

Author Information

  1. School of Medicine and Medical Science, Conway Institute, University College Dublin, Ireland

Publication History

  1. Published Online: 12 DEC 2014
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Abstract

Clustal Omega is a package for making multiple sequence alignments of amino acid or nucleotide sequences, quickly and accurately. It is a complete upgrade and rewrite of earlier Clustal programs. This unit describes how to run Clustal Omega interactively from a command line, although it can also be run online from several sites. The unit describes a basic protocol for taking a set of unaligned sequences and producing a full alignment. There are also protocols for using an external HMM or iteration to help improve an alignment. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • Clustal Omega;
  • multiple sequence alignment;
  • iteration;
  • HMM;
  • hidden Markov model
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