Unit

UNIT 7.14 Analyzing Gene Expression Data from Microarray and Next-Generation DNA Sequencing Transcriptome Profiling Assays Using GeneSifter Analysis Edition

  1. Sandra Porter1,2,
  2. N. Eric Olson2,
  3. Todd Smith2

Published Online: 1 SEP 2009

DOI: 10.1002/0471250953.bi0714s27

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Porter, S., Olson, N. E. and Smith, T. 2009. Analyzing Gene Expression Data from Microarray and Next-Generation DNA Sequencing Transcriptome Profiling Assays Using GeneSifter Analysis Edition. Current Protocols in Bioinformatics. 27:7.14:7.14.1–7.14.35.

Author Information

  1. 1

    Digital World Biology, Seattle, Washington

  2. 2

    Geospiza, Inc., Seattle, Washington

Publication History

  1. Published Online: 1 SEP 2009
  2. Published Print: SEP 2009

Abstract

Transcription profiling with microarrays has become a standard procedure for comparing the levels of gene expression between pairs of samples, or multiple samples following different experimental treatments. New technologies, collectively known as next-generation DNA sequencing methods, are also starting to be used for transcriptome analysis. These technologies, with their low background, large capacity for data collection, and dynamic range, provide a powerful and complementary tool to the assays that formerly relied on microarrays. In this chapter, we describe two protocols for working with microarray data from pairs of samples and samples treated with multiple conditions, and discuss alternative protocols for carrying out similar analyses with next-generation DNA sequencing data from two different instrument platforms (Illumina GA and Applied Biosystems SOLiD). Curr. Protoc. Bioinform. 27:7.14.1-7.14.35. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • gene expression;
  • microarray;
  • RNA-Seq;
  • transcriptome;
  • GeneSifter Analysis Edition;
  • next-generation DNA sequencing