UNIT 11.1 Large Scale Sequencing

  1. Lincoln Stein

Published Online: 1 AUG 2003

DOI: 10.1002/0471250953.bi1101s02

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Stein, L. 2003. Large Scale Sequencing. Current Protocols in Bioinformatics. 2:11.1:11.1.1–11.1.3.

Author Information

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: MAY 2003

This is not the most recent version of the article. View current version (1 DEC 2011)


DNA sequencing and assembly is the Philosopher's Stone of bioinformatics, where the raw material of wet lab enzymatic chemistry is transformed into the bits and bytes of sequence information. The sequence information becomes the foundation for extensive downstream analyses. This unit provides a brief overview of the sequencing process. It describes several software programs typically used for base calling, assembling, and finishing sequence data such as STADEN, Phred, Phrap, and Consed.